Protein Crystallography and Biophysics Centre (BiophysX)
Institute of Structural and Molecular Biology (ISMB)
Birkbeck College / University College London

Circular Dichroism Jasco J-720

Circular dichroism is the property of chiral molecules to absorb the right and left components of circularly polarized light to a different extent. CD spectroscopy measures this differential absorbance.
Nearly all biological macromolecules are chiral and lend themselves to CD experiments. For example the secondary structure content of proteins can be analysed as alpha helices, beta strands and random coil have characteristic spectra. The temperature dependence of the CD signal gives information about protein stability and folding.
Our Jasco spectrometer gives publication quality scans down to 190 nm wavelength enabling quantitative secondary structure analysis. It is equipped with a thermostatted sample chamber for melting experiments.

Sample requirements
  • Cuvettes: Several quartz length cuvettes with cell path 0.01-1cm

  • Filling volumes 26 μl for 0.1 mm, 700 μl for 1mm cuvette

  • Temperature range: 4 - 90oC
  • Sample concentration: 0.05 - 2 mg/ml, depending on the path length


Please contact us for detailed protocols and planning your experiment.

ISMB Protein Crystallography and Biophysics Centre, Birkbeck, University of London
Last modified April 2021