Protein Crystallography and Biophysics Centre (BiophysX)
Institute of Structural and Molecular Biology (ISMB)
Birkbeck College / University College London

Plate Reader VarioSkan LUX
(ThermoFisher Schientific)

Plate Reader
The Varioskan plate reader covers a wide variety of needs for research offering features that can save time and reduce common errors in the lab. The instrument has an automated dynamic dynamic range selection adjusting the optimal reading range based on signal intensities. In addition, built-in safety controls, alert for potential errors before they happen.

 Plate types: 6-384 well plates
 Wavelength Selection: Double monochromators
 Wavelength Range: 200 - 1000 nm
 Plate types: 6 - 384 well plates for bottom reading (up to 1536 for top reading)
 Wavelength Selection: Double excitation and emission monochromators
 Wavelength Range 200 - 1000 nm (excitation) , 270 - 840 nm (emission)

Time resolved fluorescence
 Plate types: 6-1536 well plates
 Wavelength selection: Filters (spectral scanning with double excitation and emission monochromators)

 Plate types: 6-1536 well plates
 Wavelength selection: Direct or filters (spectral scanning with double monochromators)
 Wavelength range: 360 - 670 nm

 Plate types: 6-1536 well plates
 Wavelength selection: Filters
  Excitation wavelength range: Fixed 680 nm
 Emission wavelength range: 400 - 660 nm

 Plate types: 6-1536 well plates
 No. of dispensers: Two
 Dispensing volume: 2–5,000 μl, in 1 µl increments (1 ml syringe) 

Incubator: From ambient to 45 °C
Shaker: Orbital

Measurement modes: End-point, kinetic, spectra, multipoint and kinetic spectra
Measurement speeds: 15s for 96-well plate, 45s for 384 well plate (minimum times)

Available filters

1. 480 nm Emission for BRET donor using wtRluc
2. 515 nm Emission for BRET2 acceptor using GFP2
3. 530 nm Emission for BRET acceptor using eYFP
4. 571 nm Emission for AlphaScreen measurement
5. 610 nm Emission for Chroma-glo luciferase CBR assay

Please contact us for detailed protocols and planning your experiment.

ISMB Protein Crystallography and Biophysics Centre, Birkbeck, University of London
Last modified April 2021