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Protein
Crystallography and Biophysics Centre
(BiophysX) Institute of Structural and Molecular Biology (ISMB) Birkbeck College / University College London |
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| The protein
crystallography laboratory is equipped for the set
up of crystallization trays, crystal monitoring
and harvesting. In detail: Liquid handling equipment: Formulator Liquid handler (Formulatrix). Applications: set-up custom crystallization screens in any crystallization plate or deep well plate. Can handle volumes from few micro-liters up to milliliters.   Hydra-96 Micro dispenser (Art Robbins). A 96 syringe liquid handler for setting up 96-well crystallisation plates from 96-deep well blocks. MultiPROBE II liquid handler (Perkin Elmer). Applications: Transforming crystallisation screens from tubes to deep well plates and setting up four corner optimisation screens. Liquidator 96 (Mettler Toledo). A manual dispenser alternative to Hydra-96. It's faster as it doesn't require washes, however is less accurate. Micro dispenser Mosquito LCP (SPT Labtech). It's our main instrument for setting up crystallization trays in a HTP mode. There are a lot of options and protocols for several applications including the set up of LCP plates. Imaging UVEXps256 (Swissci). The imaging system is operational at the 20oC crystallization room. It is equipped with 5x 10x & 20x bright-field lenses, UV and polarisers. It works with several plates , including hanging drop plates. Remote monitoring is accessible from any web browser inside the College or through Birkbeck VPN for external users. Desktop Minsterl UV and Gallery 160 Plate hotel (Rigaku). The system is not supported anymore, however it is still functional and is working in the 4oC crystallization room.   Microscopes Leica M165 microscope with imaging camera and monitor (for high res photos). Includes a polariser Leica M3Z microscope Nikon MSZ-2B microscope (3x). Including polarisers X-ray equipment (for sale) Rigaku MM007HFM with Saturn 944 CCD. The equipment is obsolete and it is not used anymore. Synchrotron related equipment The lab is equipped with several dry-shipper dewars for sending crystals to synchrotrons. We have a well organized bench with all the necessary cryo-tools, containers and pucks for efficiently harvesting crystals. |
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